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Folic acid (Folate)

What is folic acid?

Folic acid and folate are the forms of vitamin B9 which we used for treatment of certain types of anemia and pregnancy complications. Many foods such as leafy vegetables, okra, asparagus, certain fruits, beans, yeast, mushrooms, animal liver and kidney, orange juice, and tomato juice are the main sources of folate.  Folate or folic acid supplement which we used widely in our daily life has very low side effects. In chemistry, folate refers to the conjugate base of folic acid. In biochemistry, folates are the class of biologically active compounds related to folic acid. It is pteroylglutamic acid but the folic acid structure also contains three or seven glutamic acid residues.

Animal tissue contains an enzyme that hydrolysis naturally occurring pteroylpolyglutamic acids to pteroylglutamic acid and free glutamic acid.

Structure of folic acid

The structure of folic acid which contains 2-amino-4-hydroxypteridine-6-carboxylic acid, p-aminobenzoic acid, and glutamic acid is given below the picture,

Folic acid structure and uses of folate or vitamin B9 in anemia or benefit of folic acid in pregnancy

The structure of folic acid was elucidated by Angier in 1946. The alkaline hydrolysis of the Lactobacillus casei factor (folic acid) in the absence of oxygen gives two molecules of D-glutamic acid. On other hand, alkaline hydrolysis of Lactobacillus casei factor in the presence of air or oxygen gives 2-amino-4-hydroxypteridine-6-carboxylic acid, p-aminobenzoic acid, and three molecules of glutamic acid residues.

The structure of 2-amino-4-hydroxypteridine-6-carboxylic acid, p-aminobenzoic acid has been identified by the ultraviolet-visible spectrum of such compounds. The presence of amino group and hydroxyl group in 2-amino-4-hydroxypteridine-6-carboxylic acid can be identified by oxidation with chlorine water followed by hydrolysis with hydrochloric acid.

Folic acid deficiency

Folate is an important supplement during cell division, fetal growth, and organ development. Folate or folic acid deficiency reduces DNA synthesis and cell division. Folate deficiency mostly impacts pregnant women during childbirth. Therefore, folate supplements is must intake during pregnancy to reduce the chance of birth defects. The other health problems caused by folic acid or folate deficiency are heart disease, stroke, deficiency of B12, cancer, brain defects, etc.

Uses of folic acid

Folate is found in several foods such as leafy vegetables, okra, asparagus, certain fruits, beans, yeast, mushrooms, animal liver or kidney, orange juice, and tomato juice but we also used folic acid supplements or tablets in our daily life. The supplement or tablets may come in different forms such as L-methyl folate, levomefolate, and methyltetrahydrofolate. The supplements of folates are essential medicines according to the World Health Organization (WHO).

  • These supplements are used to increase folate levels in our bodies. Such types of low folate levels are caused by poor diet, alcoholism, pregnancy, liver, and kidney disease affect our health in several ways.
  • It is very effective in the treatment of certain types of anemia.
  • It helps to produce and maintain new cells in our body. It also reduces the change of DNA which causes cancer.
  • It is also used with iron to prevent birth defects. We used folic acid supplements for pregnant women in their daily food or tablets prescribed by gynecologists.
  • It is used to increase the brain health of adults. Low folate levels in the blood cause poor brain function which increases the risk of mental health disorders.
  • It also improves heart health to reduce the risk of heart failure or attack.
  • Supplement which contains folic acid is also beneficial for diabetes or kidney disease patients and helpful for fertility.


According to RDA recommendation, folic acid can intake 400 micrograms (mcg) of dietary folate equivalents (DFE) by adults, 600 mcg of DFE by pregnant women, and 500 mcg of DFE by breastfeeding women per day.

Side effects

Folate in foods or supplements is beneficial for human health in several ways but it has also side effects. If you have any unusual effects after intake of folate supplement, tell your doctor or pharmacist promptly.

The most common side effects after taking folate supplement are rash, itching, nausea, loss of appetite, bloating, gas, stomach pain, bitter taste in your mouth, confusion, trouble concentrating. sleep problems, etc. High folic acid intake may cause B12 deficiency, cancer, and negative effects during pregnancy. The overdose folate or folic acid supplement causes trouble bathing or may even die.