Analytical Chemistry Techniques

Analytical chemistry is the branch of science concerned with the analysis techniques and determination of matter. Identification of a substance, elucidation of its structure, and quantitive analysis of its composition are covered in modern analytical chemistry.

Analytical chemistry techniques, instruments and examples for quantitative analysis

The most difficult task for analytical chemists is to explain the exact branch of analytical works. Commonly, we can say, it is the branch of science where a large number of research workers have working for its development.

For example, all chromatographic or electrophoresis techniques were developed by biochemists or biological scientists but mass spectrophotometry was discovered by physicists. A large number of journals use these techniques for inorganic, organic, biochemistry, and forensic science analysis. They do not like to call themselves analysts or analytical chemists for various reasons.

Analytical Techniques

The techniques used in the field of analytical chemistry for the determinations of analytes or samples are mainly two types such as classical technique and instrumental technique.

Classical Techniques

In the classical technique, we used to check the presence or absence of a particular component in a given analyte in the lab. For example, the Kastle-Meyer test is used to identify the presence of hemoglobin in the given analyte.

Flame Tests

Flame tests are examples of classical techniques which is used to check the presence of specific elements in an analyte. When we expose the sample to a flame, the colour of the flame is changed.

Flame test for chemical analysis in analytical chemistry

Flame Tests Experiment

  1. Chemically pure hydrochloric acid (HCl) is taken in a watch beaker.
  2. The top end of a clean platinum wire moist with HCl is held on a non-Luminus Bunsen flame.
  3. The process is repeated till the flame becomes colurless. The platinum wire is again moist with concentrated HCl.
  4. A trace of the solid sample is taken at the tip of the wire and held on the base of the non-luminous flame.

The colour change of the flame in the naked eye and the double cobalt blue glass of a particular sample is given below the table,

Observation Inference
Colour in the naked eye Colour through double cobalt blue glass
Golden yellow Colorless Sodium (Na+)
Violet Crimson red Potassium (K+)
Brick red Light green Calcium (Ca+2)
Crimson red Purple Strontium (Sr+2)
Yellowish green Bluish-green Barium (Ba+2)
Green flame Copper
Bluish white Lead, arsenic, antimony bismuth compounds
Bluish-green Tin compounds

Analytical Chemistry Instruments

Different types of spectroscopic and electronic instruments are used in analytical chemistry for quantitative or qualitative analysis of samples.

  • Electrophoresis or gel electrophoresis instruments are used widely for the purification or separation of proteins, amino acids, enzymes, DNA, and RNA in biochemistry.
  • Spectroscopy involves the measurement and analysis of electromagnetic radiation of given atoms or molecules present in the sample.
  • A common instrument that we use widely in analytical chemistry is the electrochemical cell. It is used mostly for the purification and analysis of metals.

Quantitative Analysis

In analytical chemistry, the amount of sample and range of relative amounts of constituents are important characteristics of quantitative analysis. It can be divided into three categories such as macro, semi-micro, and micro.

  • In macro methods, we can analyze the sample whose weight is greater than 0.100 g.
  • In semi-micro, we can analyze the sample whose weight is between 0.100 to 0.10 gm.
  • In micro, we can analyze the sample whose weight is less than 0.10 g.
  • A sample whose weight is less than 0.002 g is called a submicro or ultramicro sample.

The important steps for quantitative analysis are,


It should represent the mass of the material. It can provide the martial in reasonable pure form.


The separation technique is used for the conversion of desired constituents of the sample to a measurable form. The selection of the separation technique for a specific situation depends on a number of factors. Such selection is required for accuracy and precision.

Measurement of Desired Constituent

Any physical or chemical property can be used for qualitative identification and quantitative measurement of the sample. If the property is specific and selective for measurement, then separation and pretreatment of the sample can be minimized.

Calculation and Interpretation of Analytical Data

An analysis is not complete until the results are expressed in such a manner that can be understood by all. In recent years greater attention has been given to statistical data on analytical chemistry. It can establish a new branch of science which is termed Chemometrics.

Applications of Analytical Chemistry

No other branch of science finds such extensive application as analytical chemistry. It has numerous applications in various fields of chemistry such as inorganic, organic, physical, and biochemistry.

It has also wide applications in other fields of science such as environmental science, agricultural science, biomedical or drug research, forensic science, and space research.

  • In environmental science, analytical chemistry techniques are used for monitoring sulfur dioxide and carbon dioxide.
  • Analysis of dissolved oxygen and chlorine from water can be carried out by potentiometry and colorimetry.
  • For the analysis of pesticides and insecticides from crops, we used the gas and high-performance liquid chromatography technique in analytical chemistry.
  • Spectrophotometry is another instrument used for the analysis of micronutrients such as iron, copper, zinc, molybdenum, boron, and manganese in the soil sample.
  • In the field of electronics, analytical chemistry is used for the analysis of traces of elements such as silicon or germanium in semiconductors and transistors.
  • In the field of oceanography, earth science, planetary science, and analytical chemistry are extensively used for the analysis of seawater, blastic rocks, and lunar samples.

Analytical Chemistry Topics

Atomic Emission Spectroscopy
Chromatography Capillary Electrophoresis
Column Chromatography
Gas Chromatography Gel Permeation Chromatography
High Performance Liquid Chromatography (HPLC)
Ion Exchange Resin Ion Exchange Chromatography
Mass Spectrometry
Paper Chromatography Polarimetry
Size Exclusion Chromatography Supercritical Fluid Chromatography
Thin Layer Chromatography

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